Wiley Higher Education Supplementary Website

	Questions
	Animations
	Further Links
	Figures

Web links Chapter 1 - DNA structure and function
www.nobel.se		Read the achievements and autobiographies of Nobel Prize winners
www.blc.arizona.edu/molecular_graphics /dna_structure/dna_tutorial.html		A static tutorial in the structure of DNA and its components
molvis.sdsc.edu/dna/index.htm		An interactive tutorial in the structure of DNA and its components (requires the CHIME plug-in)
www.dna50.org/main.htm		Various DNA-related topics, including the text of Watson and Crick's 1953 Nature paper
www.johnkyrk.com/DNAanatomy.html		Animations of DNA structure, replication, transcription and translation
www.dnai.org/index.html		Interactive site containing various DNA-based tutorials
Chapter 2 - Basic techniques in gene analysis
rebase.neb.com/rebase		Comprehensive database of restriction enzymes and their recognition sites
rebase.neb.com/rebase		Comprehensive database of restriction enzymes and their recognition sites
www.cellbio.com		Informational resource for cell and molecular biology
micro.nwfsc.noaa.gov/protocols		Molecular biology protocols
www.life.uiuc.edu/molbio /geldigest/electro.html		Loading and running an agarose gel
web.utk.edu/~khughes/GEL/index.htm		Loading and running a gels in pictures
Chapter 3 - Vectors
www.promega.com		Commercial site describing various vectors
www.stratagene.com		Commercial site describing various vectors
www.bdbiosciences.com/clontech		Commercial site describing various vectors
www.invitrogen.com		Commercial site describing various vectors
www.orbigen.com/protocols/Vectors.html		Links to a variety of vector information and methods
Chapter 4 - PCR
highveld.com/f/fpcr.html		Information and links on all aspects of the polymerase chain reaction
web.uct.ac.za/microbiology /pcroptim.htm		Technical consideration of PCR reaction conditions
alces.med.umn.edu/rawtm.html		Oligonucleotide annealing temperature determination
www.dnalc.org/shockwave /pcranwhole.html		PCR animation
alces.med.umn.edu/xprimerinfo.html		Design of PCR primers
allserv.rug.ac.be/~avierstr /principles/pcr.html		The basics of PCR
Chapter 5 - Cloning a gene
www.guthrie.org/AboutGuthrie /Research/CDNA		The Guthrie cDNA resource centre
www.hgmp.mrc.ac.uk		IMAGE cDNA clones
www.fhcrc.org/education/courses /cancer_course/basic/approaches /libraries.html		A relatively simple description of cDNA and genomic libraries
Chapter 6 - Gene identification
www.dyax.com/phage/phage.asp		Phage display and how it works
www.uib.no/aasland/two-hybrid.htm		A variety of links and explanations of the two-hybrid system
depts.washington.edu/sfields /yplm/data/index.html		Two hybrid interaction map of proteins in yeast
www.molsci.org/brentlabweb /interactiontrap.html		Detailed information on the two hybrid interaction trap
Chapter 7 - Creating mutations
www.protocol?online.org/prot /Molecular_Biology/DNA/Mutagenesis/		Links to a variety of mutagenesis protocols
www.promega.com/pnotes/61 /6222_12/6222_12_core.pdf		Description of different primer extension mutagenesis techniques
www.stratagene.com/intros/quickchange.htm		Quikchange mutagenesis
labtools.stratagene.com /QC/QCmaster.php		Design of Quikchange mutagenesis primers
Chapter 8 - Protein production and purification
www.cf.ac.uk/biosi/staff /ehrmann/tools/geneexpression.htm		Discussion of factors influencing protein production in E. coli
www.bdbiosciences.com/clontech /tet/index.shtml		The Tet-on and Tet-off systems
www1.qiagen.com/literature /handbooks/int/proteinpurification.aspx		Description of the his-tag and how it is used to purify proteins
www.neb.com/neb/products /pfp/pfp.html		Maltose binding protein as a purification tag
www.neb.com/neb/products /pfp/6900.html		The IMPACT protein production and purification system
Chapter 9 - Genome sequencing projects
www.ornl.gov/TechResources /Human_Genome/project/info.html		DNA sequencing and the human genome project
www.dnalc.org/shockwave/cycseq.html		Cycle sequencing animation
www.hgmp.mrc.ac.uk/Embnetut/Gcg/		An introduction to DNA sequence analysis and bioinformatics
www.genome.wisc.edu		Escherichia coli genome database
genolist.pasteur.fr/SubtiList		Bacillus subtilis genome database
genome-www.stanford.edu/Saccharomyces		Saccharomyces cerevisiae genome database
www.wormbase.org		Caenorhabditis elegans genome database
flybase.bio.indiana.edu		Drosophila melanogaster genome database
www.arabidopsis.org		Arabidopsis thaliana genome database
www.informatics.jax.org		Mus musculus genome database
www.ncbi.nlm.nih.gov/genome /guide/human		Homo sapiens genome database
Chapter 10 - Post-genome analysis
www.genhunter.com		Differential display methods and explanation
cmgm.stanford.edu/pbrown		Pat Brown's microarray information at Stanford
www.affymetrix.com		Affymetrix system for microarray analysis
www.upstate.com/misc/protocols.q.prot.e.chips /Chromatin+Immunoprecipitation ++ChIPs++Assay+Kit		Chromatin immunoprecipitation
web.mit.edu/mmcmanus/www /home1.2files/RNAi.htm		RNA interference
Chapter 11 - Engineering plants
helios.bto.ed.ac.uk /bto/microbes/crown.htm		The biology and control of crown gall
www.usask.ca/agriculture /plantsci/classes/plsc416 /projects_2002/christine/ webpage/		Chloroplast transformation
helios.bto.ed.ac.uk /bto/microbes/bt.htm		Bacillus thuringiensis and its use as an insecticide
www.monsanto.com		Monsanto, which produces a variety of genetically engineered crops
www.geneticsaction.org.uk		Genetic Engineering Network - opposed to the introduction of GM technology
Chapter 12 - Engineering animal cells
www.stanford.edu/group /virus/adeno/adeno.html		Adenovirus resources
www-micro.msb.le.ac.uk /3035/Retroviruses.html		A concise, but very good description of retroviruses and their life-cycle
www.animalbiotechnology.org		The Federation of Animal Science Societies
www.nuffieldbioethics.org/home		Nuffield Council on Bioethics
Chapter 13 - Engineering animals
www.roslin.ac.uk		The Roslin Institute, where Dolly was cloned
www.ppl-therapeutics.com		PPL Therapeutics
www.time.com/time /newsfiles/cloning		A Time Magazine feature on cloning
stemcells.nih.gov		Information from the National Institutes of Health on stem cells
www.ornl.gov/TechResources /Human_Genome/medicine/genetherapy.html		Gene therapy
www.accessexcellence.org/AB /IWT/Gene_Therapy_Overview.html		An overview of gene therapy


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